Pioneer SPR System
Simplified Kinetic Characterization – With OneStep injections, users can obtain accurate kinetic and affinity values from a single analyte concentration, eliminating the need to run multiple analyte titrations and reducing both time and errors associated with dilution preparation. NextStep injections provide a simple workflow for characterizing competitive binders against a ligand to capture the mechanism of action.
High Sensitivity – Pioneer systems provide the sensitivity required to characterize a wide range of analyses, from biologics to small-molecule fragments, with a high degree of confidence.
- Categories: Protein Analysis | OneStep SPR Systems |
Pioneer SPR systems provide high-quality kinetic data (ka, kd) and affinity measurements (KD) for characterizing a wide range of biomolecular interactions, from small-molecule fragments to biologics. OneStep SPR injections enable faster assay workflows, as a single analyte concentration is sufficient for accurate kinetic and affinity determination.
Three-Channel, Fully Automated SPR System
With three detection channels, two target proteins or ligands can be analyzed simultaneously while a third surface serves as a reference channel.
Up to 72 Hours of Unattended Operation
The system supports a variety of sample container formats, including a capacity of up to two 384-well plates, providing approximately 72 hours of unattended runtime for large-scale binding screens.
Rapid Data Analysis with QDat Software
Pioneer SPR systems are equipped with integrated, industry-proven data analysis software based on the Scrubber and Clamp platforms. Raw data collected across multiple runs, days, and sensors can be combined and analyzed using binding algorithms such as single-site, multi-site, mass transport, and irreversible inhibitor analysis models.
Gain More Information from Less Data
Because an analyte gradient is generated during OneStep injections, a diffusion coefficient can be calculated, providing users with information not only about kinetics and affinity but also an assessment of aggregation from a single injection.
Robust Fluidics Design
The Pioneer fluidics system is constructed from inert materials such as PEEK, ceramic, and Tefzel, ensuring durability and low maintenance requirements.
Applications
Characterization of High-Affinity Biological Interactions
Target binding characterization is a critical analytical step for selecting highly specific biologics with high affinity (KD < 1 nM), regardless of molecular type. Kinetic analysis provides a more detailed understanding of the association and dissociation components that contribute to overall affinity. For example, two lead compounds may exhibit similar affinity values (KD), but differences in association and dissociation rate constants can help predict which candidate will perform better in vivo. Accurate analysis of these kinetic rate constants is essential for lead selection and for predicting the efficacy of protein therapeutics.
With next-generation SPR injections, the Pioneer SPR system determines kinetics and affinity in a single step, significantly improving the efficiency of the characterization process compared to traditional SPR methods. The next-generation OneStep gradient injection technology included in the Pioneer platform greatly accelerates affinity characterization while maintaining high accuracy and confidence in the results.
Characterizing Irreversible Inhibitors and Measuring Covalent Engagement
Most small-molecule inhibitor assays evaluated using label-free, real-time biosensor technologies involve reversible interactions characterized by commonly used kinetic models. However, a significant proportion of therapeutic enzyme inhibitors on the market function through covalent modification of their targets.
Pioneer is an SPR platform compatible with regenerable Streptavidin biosensors, enabling oriented capture of protein targets and measurement of the efficiency of covalent inhibitors binding to those targets. The Pioneer system’s irreversible inhibitor application method can be used to determine covalent commitment (Cc) of an inhibitor compound, providing an important metric for evaluating irreversible inhibitors.