GlyM Kit
Designed for the analysis of both crude and purified samples:
- No purification or digestion required
- Relative mannose content screening and classification of samples into high, medium, or low groups
- High-throughput analysis of up to 96 samples in parallel in less than 1 hour
- Categories: Protein Analysis | Biosensors and Kits |
Glycosylation, a critical post-translational modification, is an important critical quality attribute (CQA) that affects product safety and efficacy and must be monitored during the development of new biologic drugs. Among various glycan structures, mannose is particularly important because it can influence the plasma clearance of a protein and reduce its in vivo half-life, potentially impacting therapeutic efficacy.
Although process conditions, media, and feed formulations are critical factors in optimizing protein mannosylation, optimal cell line selection can be a limiting step. Early screening for mannose content can facilitate the selection of clones that produce proteins with desirable glycan profiles during cell line development (CLD).
The Octet® GlyM Kit enables high-throughput screening of mannose content in crude cell culture samples during the early clone selection stages of the CLD process. When combined with titer analysis, it accelerates the identification of top-performing cell lines that produce proteins with the most desirable glycan (mannose) profiles. Only the most suitable clones are advanced for further optimization. Reliable data generated at this early stage support shorter development timelines and help prevent downstream failures.