Blue Trisacryl® M
Blue Trisacryl® M is an affinity resin used for the purification of enzymes, interferons, and certain coagulation factors, as well as for albumin removal. The resin utilizes Trisacryl®-based beads carrying a blue dye ligand.
- Categories: Process Chromatography | Chromatography Consumables | Chromatography Resins | High-Capacity Affinity Resins |
Blue Trisacryl® M dye affinity chromatography resin is used for the purification of a wide range of enzymes and proteins, including kinases, interferons, and certain coagulation factors. It is also used for albumin purification or albumin depletion in samples intended for proteomics research.
Blue Trisacryl® M resin is based on Trisacryl®, a macroporous non-ionic synthetic polymer on which the blue dye is covalently immobilized. Due to its structural similarity to nucleotide cofactors (ADP, NADP), the blue dye interacts biospecifically with certain proteins. Other proteins, such as albumin and interferons, bind through a combination of hydrophobic, electrostatic, and π-π interactions.
Features and Benefits
- Unique multimodal interaction mechanism
- Low ligand leakage
- Multiple applications from laboratory scale to manufacturing scale
Applications
- Enzymes requiring NAD as a cofactor (kinases, dehydrogenases, phosphatases)
- Other enzymes (sulfatases, RNA polymerases, monooxygenases, oxidoreductases)
- Plasma-derived albumin
- Plasma transferrin
- α1-acid glycoprotein, α-fetoprotein, α1-proteinase inhibitor
- Serine proteases
- Interferons
- DNA antibodies
- Recombinant vaccine purification processes